Metabolite profiling of Calvin cycle intermediates by HPLC-MS using mixed-mode stationary phases

Stability of stationary solutions in models of the Calvin cycle

In this paper results are obtained concerning the number of positive stationary solutions in simple models of the Calvin cycle of photosynthesis and the stability of these solutions. It is proved that there are open sets of parameters in the model of Zhu et. al. [15] for which there exist two positive stationary solutions. There are never more than two isolated positive stationary solutions but...

Photosynthesis by isolated chloroplasts. Reversal of orthophosphate inhibition by Calvin-cycle intermediates.

1. The orthophosphate inhibition of photosynthesis by isolated spinach chloroplasts can be reversed by 3-phosphoglycerate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, fructose 6-phosphate and fructose 1,6-diphosphate. 2. Metabolically related compounds such as ribulose 1,5-diphosphate, glucose 6-phosphate, 6-phosphogluconate and phosphoenolpyruvate are ineffective. 3. The kinetics o...

Metabolite profiling with HPLC-ICP-MS as a tool for in vivo characterization of imaging probes

Background Current analytical methods for characterizing pharmacokinetic and metabolic properties of positron emission tomography (PET) and single photon emission computed tomography (SPECT) probes are limited. Alternative methods to study tracer metabolism are needed. The study objective was to assess the potential of high performance liquid chromatography - inductively coupled plasma - mass s...

Separation and detection of macrolide antibiotics by HPLC using macrolide-imprinted synthetic polymers as stationary phases.

preparation of polymeric materials containing recognition sites of predetermined specificity. The resulting polymers retain a memoryfor the print molecule and can be used in the HPLCmodefor separation of structurally similar substances, and (in case of single enantiomer imprinting) resolution of the enantiomers of the print species10). Polymerisation of monomers, which are selected for their ab...

Determination of Urinary Trimethylamine and Trimethylamine N-oxide by Liquid Chromatography-Tandem Mass Spectrometry Using Mixed-Mode Stationary Phases